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Title |
Affiliation |
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Luis A. Colon
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Ultrahigh Pressure Chromatography (UPLC) |
SUNY @ Buffalo, Dept. of Chemistry |
Liquid chromatography (LC) continues to be an indispensable technique in
most analytical laboratories, with a remarkable impact on chemical
analysis. It is difficult
to imagine an analytical laboratory without state-of-the-art HPLC
equipment. Column
technology is one of the most investigated research topics in separation
sciences; this is not surprising since it is within the column that the
chromatographic processes take place, making the column the “heart”
of the separation system. The
use of HPLC columns packed with small particle diameter leads to an
increase in the speed of the separation, with a concomitant increased in
separation efficiency. To reduce the back pressure requirements that accompany the
decreased in particle diameter, the length of the column has
traditionally been shortened. Recently,
however, the use of very high pressures, beyond the traditional 6,000
psi, has gained considerable attention as a means to drive the mobile
phase through columns packed with small diameter particles (<2 um). We are investigating the use of columns with inner diameters
of about 1 mm packed with small particles, under relatively
high-pressure conditions (>15,000 psi).
These columns have been operated at such high pressures and do
not appear to be more problematic than using regular 4.6 mm i.d. columns
under typical HPLC pressure conditions.
Our presentation will discuss the advantages and need for
ultrahigh pressures, as well as our most recent investigation using
ultrahigh pressure LC.
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Dwight Tshudy
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Bio-Diesel, From French Fries to Rabbits |
Gordon College, Dept. of Chemistry
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The talk of alternative energy is increasing, especially as gasoline and
natural gas prices increase. There
are many options that are being investigated as ways of offsetting the
dependency on fossil fuel based energy sources.
These range from energy sources such as solar, wind, fuel cells
and alternative fuels. Biodiesel
is alternative fuel that comes from renewable sources.
What is considered biodiesel? How is it made?
How similar is it to petrol diesel?
Why are we interested in using biodiesel? How are fuels such as biodiesel tested? These questions and other will be presented and discussed.
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Troy. D. Wood
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LC-MS - Nuts and Bolts of Instrumentation |
SUNY @ Buffalo, Dept. of Chemistry
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The combination of liquid chromatography with
mass spectrometry (LC-MS) has proved a revolutionary tool in the fields
of biomedicine, pharmaceutical sciences, and environmental protection.
In this presentation, the history of the development of LC-MS
from its roots to the present will be presented.
A focus on the fundamentals of the ionization sources, mass
analyzers, and interfaces that have made LC-MS such a broadly applied technique will be
discussed.
|
Robin DiFrancesco |
Biological Matrix Suppression Concerns in LC-tandem MS
Analytical Methods |
SUNY @ Buffalo, Dept. of Pharmacy
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Liquid chromatography tandem mass spectrometry (LCMS) analytical methods
are practiced as common state-of-the-art laboratory methods in an array
of industrial, government, and academic laboratories.
Our laboratory specializes in methods that support clinical
trials in true patient populations. Consequently, the heterogeneity of the samples analyzed as
well as numerous concomitant medications taken create special issues to
consider when developing and validating quantitative LCMS methods for
measuring specific analytes of interest.
This short presentation will review the concepts of matrix ion
suppression [and enhancement], highlight existing approaches to
investigating matrix suppression effects, and present findings from our
laboratory’s approach to exploring matrix effects while validating
several LCMS methods in biological matrice
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Daniel
Sudlik |
Using Enterprise Content Management to Integrate
Laboratory and non-Laboratory Information |
Agilent
|
Information management in the laboratory is becoming increasingly
complex due to the need to sort through a stream of email, powerpoint
slides, PDF files and other data sources, along with standard
instrumentation data files like chromatograms and spectra. ECM
(Enterprise Content Management) offers a unique tool to collate multiple
data sources into a usable format that allows a scientist to easily
locate or store information.
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Timothy
C. Schunk |
A Systematic Approach to the Development of
Gradient HPLC Copolymer Composition Separation Methods |
Eastman Kodak Company
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HPLC copolymer composition separation methods can be difficult and time
consuming to develop. Based on the available literature, it is often
unclear to the non-expert what path will provide the needed separation
in an efficient manner. The general experience of chromatographers often
leads to confusing results because of the differences in solution
thermodynamics and kinetics of polymers relative to small molecules. The
work presented here provides the foundation for a systematic approach to
the development of gradient HPLC copolymer composition methods
emphasizing adsorption separations. An organized series of steps are
used to define a linkage between the information required and the
chemical structure and solubility behavior of copolymers. A method
development plan is outlined to define an initial method, and it is
refined to provide the needed separation. Throughout, the focus is
maintained on the chemical information required rather than the
separation type employed (reversed-phase, normal-phase, etc.)
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| Witold Lawrynowicz |
Ion Chromatography Analysis of Insoluble Samples |
Xerox Corporation
|
Many types of samples can be difficult to analyze by ion chromatography.
While some aqueous-insoluble samples can be prepared by
solvent/non-solvent preparations such as in dimethylformamide and water,
others are not soluble at all. Although surface-extracted ions are
relevant and can be determined, it is still often desired to determine
the total ion content of some samples. In these cases, insoluble
samples such as pigments can be combusted using an oxygen bomb
apparatus. Following combustion, the residue is dissolved in
typical aqueous IC solutions. Using this technique, the levels of
halogens, sulfate and phosphate can be quantitatively determined.
|
Jill Hochreiter |
Conversion of a Traditional HPLC Method to UPLC Method |
SUNY @ Buffalo, Dept. of Pharmacy
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Ultra Performance Liquid Chromatography (UPLC) is
a new technology touting increased speed of analyses along with superior
resolution and sensitivity. Our
laboratory desired to incorporate a published method for several
nucleoside antiretrovirals that required an analysis time of 25 minutes
and a lower limit of quantitation of 15 ng/mL.
Initial experiments using the UPLC equipment provided a decreased
run-time of ~10 minutes. Several
comparisons within the UPLC system’s options were performed to
determine optimum parameters to gain the sensitivity desired:
use of a UV detector and a photodiode array detector, sample
injection volume using different loop sizes, and use of different UPLC
analytical columns. In
addition, we were concerned about the many concomitant medications HIV
patients might also be taking. Specifically,
would the decreased retention times create more opportunities for
interferences by other analytes? Would
the increased resolution compensate adequately?
This short presentation will provide an overview of our findings
while investigating the applicability of the UPLC technology in our
laboratory.
|
| Lee Marotta |
Optimizing Detection Limits Using Headspace Trap
Technology |
PerkinElmer LAS |
The advantages of determining volatile compounds in difficult sample
matrices via headspace have been reported for a wide range of
applications, making the technique a popular choice in many of today’s
laboratories. The necessity to enhance detection limits from what
“classical headspace” can provide is increasing.
This seminar will discuss a novel headspace sampling system that will
enable up to 100 times better detection than that provided by classical
headspace. This is possible without the need for liquid cryogen, and
will retain the convenience and performance offered by headspace
analysis systems.
Therefore, the analyst will still enjoy the excellent precision,
recovery, ease of use, and productivity (virtually no sample prep)
offered with classical headspace, and achieve up to 100 times better
detection limits. In
addition, this will enhance precision since responses will be greater;
therefore, peak integration will be more repeatable.
In addition to enhanced detection limits, this headspace trapping
technique uses a dry purge, which eliminates the majority of water and
eliminates all of the air being transfer to the analytical system while
retaining the analytes of interest for analysis.
We will demonstrate the ability to obtain enhanced detection limits of
volatile compounds in several matrices.
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| Dan Bazavan |
Fast HPLC Methodology which Increases Productivity |
Dionex
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POSTERS
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Julia Kohn |
Proteomic analysis of Pseudomonas Putida KT2440 in the
presence of Diethylstilbestrol |
RIT Proteomics lab
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The RIT Proteomics lab has been using a 2D-Gel Electrophoresis analysis
of proteins to study different carcinogens and their effects on the
protein expression in the bacteria Pseudomonas Putida. The
current screening test for a carcinogen is called the Ames Salmonella
Assay. The problem with
this assay is that it looks only at cell growth and not at protein
expression, and it also gives false negatives and false positives.
Looking at the protein expression allows for a more precise view
of whether the compounds being tested are carcinogens by seeing its
effects on the protein level. The
strain being studied is KT2440 and is grown on a 5mM Succinic acid
carbon source. The
carcinogen being studied is Diethylstilbestrol (DES).
DES is a synthetic estrogen that was given to women between the
1930s and 1970s and was believed to prevent premature births as well as
miscarriages. Later results
determined that DES actually caused a rare vaginal cancer in the women
who were born from a mother that was given DES.
DES is also one of the carcinogens that give a false negative in
the Ames Test. Analysis of
the effect of the concentration of DES on KT2440 was performed and
analyzed, showing an effect on the length of the growth curve as well as
an effect on the amount of cells that were able to grow.
Growth curves for KT2440 in the presence of .0025mM DES and
Succinic acid have been performed and also the mid-log phase of growth
was determined. Initial gels are being run to look at the changes in the
protein expression.
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