Separation Technology in Proteomics  

Presented by: Tim Wehr of

Thursday, May 9th
8:00 - 4:00

RIT Inn and Conference Center
(formerly Thruway Marriott)
5257 West Henrietta Road, Rochester, NY

Proteomics is the determination of the constellation of all proteins expressed in a cell or tissue under a given physiological state.  With the first draft of the human genome in hand, proteomics studies allow the health care industry to move forward in the validation of drug targets, in elucidation of the mechanism of drug action, and in qualifying candidates for therapeutic intervention.  However, the enormous complexity of the proteome places major obstacles in the way to achieving this task. This one-day course will discuss the separation technologies currently used in proteomic studies, how chromatographic and electrophoretic techniques are integrated, and some new technologies which may aid and simplify the task.

8:00 am             Check - In (Continental breakfast provided)

9:00 – 9:30        Introduction to proteomics

                        ·    What is proteomics and why it is important?

                        ·    Challenges in analyzing the proteome

 9:30 – 10:00    Two-dimensional gel electrophoresis: the workhorse of proteomics

                        ·    Evolution of 2D-GE and its current implementation

                        ·    Steps in a typical proteomics experiment

                        ·    Limitations of 2D-GE

 10:00 10:15        Break

 10:15 – 10:30    Overview of alternative separation technologies for proteomics

 10:30-12:00       Micro LC-MS in proteomics

                        ·    Developing a gradient micro LC-MS method

                        ·    Instrument considerations in configuring a gradient micro LC system

                        ·    Interfacing micro LC systems with atmospheric pressure ionization sources

                        ·    Differential expression mapping with reversed phase micro LC-MS-MS

 12:00 – 1:00       Lunch (provided)

 1:00 – 2:30         Capillary electrophoresis in proteomics

                        ·    Overview of CE separation modes for proteomics

                        ·    Developing capillary zone electrophoresis and capillary  isoelectric focusing
                               methods

                        ·    Interfacing CE systems with API sources

 2:30 – 2:45        Break

2:45 – 3:15        Sample preparation techniques for proteomics

3:15 – 4:00        Future directions

                      ·    Protein microarrays

            ·    Planar microscale 2-D systems

NERCDG Home Page