NERCDG Spring 2001 Training Session
May 2, 2001
Buffalo Four Points Sheraton Airport

Topic: Sample Preparation for Chromatography

Presented by: Merlin K. L. Bicking, Ph.D and Joseph Levy, Ph.D

Schedule:

Who should take this course?

If you’d like to better understand the principles behind sample preparation techniques, new and old, this course is a must. Those interested in learning about new advances in traditional techniques and in finding out what new technologies are available for sample preparation will benefit as well.

• How to better control variables in sample preparations to yield accurate, reproducible results
• How to develop reliable methods in less time with more efficiency
• New advances to use with traditional procedures and techniques
• New sample preparation technologies and their principles

You will learn how to do the best possible sample preparation. Save hours of your time and improve your results.

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Come prepared to learn that sample preparation is more than just a few "low technology" procedures. Learn about the chemical principles behind the techniques, and how an understanding of these principles will provide better results. This course will include a survey of many traditional procedures, including information on recent advances in these techniques. Several new sample preparation technologies will also be introduced.

This is not a "recipe" course limited to a particular sample type or application. This course offers a comprehensive treatment of sample preparation as an important part of every analytical method. You will learn more than just a few manipulations; you will come away with a complete understanding of what sample preparation is and how you can use it.

Students will realize the following benefits from this course:

• Better control of variables in sample preparation steps, producing more reliable results.
• Better efficiency and decision-making skills, since time is not wasted on unnecessary or lengthy manipulations
• A knowledge of what traditional techniques are available, and what recent advances make them even more efficient.
• An understanding of several "new" technologies that might also be useful.

Outline

1. Perspectives on the Importance of Sample Preparation
2. General Principles
   • Physical Changes, LeChatelier's Principle
   • Effects of Temperature, Time, Ionic Strength, and pH
   • "Like-Dissolves-Like"
   • Partitioning Equilibria
3. Traditional Procedures
   • Filtration
   • Drying Agents
   • Solvent Evaporation
   • Solvent Exchange
4. Traditional Techniques
   • Liquid-Liquid Extraction
     • Separatory funnels
     • Vectasep CLE, Mixxor
     • Other variations
     • Emulsions
   • Liquid-Solid Extraction
     • Soxhlet/Soxtec
     • Microwave-based methods
     • Ultrasonics
     • SPE
5. New Technologies
   • Membrane Disk Extractions
   • GC Sample Preparation
   • Supercritical Fluid Extraction (SFE)
   • Solid Phase Microextraction (SPME)
   • Accelerated Solvent Extraction (ASE)

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