NERCDG Spring 2000 Training Session

Systematic HPLC Method Development

Presented by Tom Jupille of LC Resources

Tuesday May 2^nd

Rochester Marriott Thruway Hotel
5257 West Henrietta Road
Rochester, NY

Tom Jupille Tom Jupille has written more than 30 papers on chromatography and related subjects. Over the past 18 years, he has taught courses on a wide range of subjects in the field of chromatography and has been involved in the development and marketing of software and training products for chromatography. He is consulting editor for LC/GC.

For details, check out Course Description.

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Systematic HPLC Method Development

May 2, 2000

Course Description

Who should take this course?
If you've ever wished that HPLC method development was more logical and methodical, this is the course for you. Attendees should have at least one year of HPLC experience in the laboratory and at least some involvement in developing new methods or troubleshooting older ones. This course is for chromatographers who want to develop better methods faster and less expensively.

What does it cover?
You will learn a powerful new approach to systematic method development.

Learn the modern approach to method development

• Define your objectives clearly. For example, your objective may be:
  - to separate and account for all impurities at the 0.1% level
  - to develop rugged, precise methods quickly and efficiently
  - to have validated methods accepted by the FDA
• Apply the best approach to separating mixtures that contain acids, bases, or isomers -- or to separating difficult samples.
• Take advantage of recent improvements in column technology to assist you in your method development efforts.

Learn how to develop a satisfactory method

• Learn to develop rugged methods that have superior separation repeatability.
• Design system suitability checks and validation into your method.
• Understand the factors that control precision.

Improve your troubleshooting skills

• Develop improved problem-solving techniques based on this new approach to method development.

Improve your understanding of the effects of chromatographic variables through the use of computer modeling

• Solve case study problems that clearly demonstrate how this methodology works.

What will I get from this course?
Using chromatographic computer modeling, you will solve a series of real-world problems that clearly demonstrate how this approach to method development works. The principles used in computer modeling are fully applicable to separations in your lab. You will learn how to do the best possible chromatography. Save hours of frustration and improve your results time after time.

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